HIV-1 Vpr Induces Adipose Dysfunction in Vivo Through Recipr(8)

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B.W.O’Malley,S.Khosla,Skeletalconsequencesofdeletionofsteroidreceptorcoactivator-2/transcriptionintermediaryfactor-2.J.Biol.Chem.284,18767–18777(2009).Acknowledgments:WethankH.Mersmann,L.A.Cowart,V.Yechoor,B.Chang,andH.J.Pownallforvaluablediscussions;J.W.Hsu,S.Hartig,P.Saha,andW.Chenforexperimentalhelp;andE.DavidsonandD.Nguyenfortechnicalassistance.WearegratefultothelateP.J.Reedsforinsightsthatguidedthisstudy.Funding:ThisworkwassupportedbyDK081553andaDevelop-mentalGrantfromtheBaylorCenterforAIDSResearchP30AI36211(toA.B.),aSeedaward(toR.V.S.),P30DK079638(DiabetesResearchCenteratBaylor),GermanResearchCouncilgrantsSFB796and643(toU.S.),theNIDDKIntramuralResearchProgram,andtheNIHOfficeoftheDirector.Authorcontributions:N.A.andD.I.performedallmousetissueandcellexperimentswithassist-ancefromT.O.;S.G.P.designedcriticalexperimentsandprovidedtechnicalguidance;R.V.S.designed,optimized,andperformedmouseinfusionstudies,plasmaderivatization,MS,andcalorimetry;T.M.P.performedVprmeasurementsbyICE;U.S.providedmousemAbsforVprdetection,synthesizedandconductedqualityassessmentofsVpr,andprovidedwild-typeandVpr-deficientHIVNL4.3;E.D.B.performedimmunohistochemistry;S.L.S.performedglucosetolerancetestsandoptimizedqPCR;J.C.andD.E.L.performedflowcytometry;M.C.R.-B.obtainedserumsamplesandclinicaldatafromuntreated/NRTI-treatedHIVpatients;F.J.performedMS;T.K.constructedwild-typeandmutantVprplasmidsandprovidedadviceonGR-relatedmethods;J.B.K.engineeredVpr-TgandprovidedadviceonPPARg-relatedmethods;A.B.con-ceivedtheproject,obtainedfunding,designedandsupervisedtheexperiments,petinginterests:J.B.K.isauthoronapatentontheuseofthemAbtoVpr.(USpatentapplicationnumber11/630,880;“MonoclonalantibodiestoHIV-1andmethodsofusingsame”.)Theotherauthorsdeclarethattheyhavenocompetinginterests.Submitted25July2013Accepted2October2013Published27November201310.1126/scitranslmed.3007148…… 此处隐藏：4217字，全部文档内容请下载后查看。喜欢就下载吧 ……